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Trainings that could fill the gap between academics and professional standards by producing skilled manpower in the field of research and diagnosis had been long awaited. Intrepid Nepal has taken an initiation to generate modern science and technology experts particularly in Molecular biology and associated fields. The training program entitled “Extensive training on Molecular and Immunological Techniques” which is tentatively scheduled for two weeks from 30th June to 17th July 2008 and covers latest molecular techniques plus immunological tests, considered the most rapid and confirmatory in the detection of pathogen of all sorts.

The training will be instructed by Ms. Elizabeth Cowan, research personnel from Harvard Medical School, United States. She will be conducting all molecular approaches and immunological techniques of modern days and will be sharing her life time experiences. The course, designed for the training is highly extensive and employ the latest knowledge and techniques including kit methods in detection and prevention of disease, comprise of following

1. PCR (Polymerase Chain Reaction)
2. ELISA (Enzyme- Linked Immuno sorbent Assay)
3. SDS PAGE (Sodium do-decilesulphate Polyacrylamide gel Electrophoresis)
4. Gel Electrophoresis
5. Quantitative Real Time PCR
6. Reagent and chemical preparation
7. Data analysis, Management and interpretation
8. Theories underlying behind each process and DNA sequencing too..
9. Many more

PCR (Polymerase chain reaction): technique that has enhanced sensitivity and accuracy will be covered in the training program. Trainees will be conducting hands on sample preparation such as Nucleic acid extraction from biological sources, pre-amplification procedures, amplification and finally post reaction analysis including verification of the product generated. Furthermore the courses include Protein extraction from different sources (Possibly from Microorganisms), analyzing them on Gel (SDS PAGE) and studying their characteristic bands against standard Protein markers (again another process for diagnosis of pathogens). They will also be taught about buffer and reagent preparation, plus their significances and limitations, whose role is immensely important in the process of experimentation.

Likewise, QPCR (Quantitative Real Time PCR): modified form of conventional PCR will also be included, at least to provide introduction on its role on quantitation of bacterial pathogens, viral load (such as HIV or HBV) in clinical samples and antiretroviral treatment efficacy, and detection of genetic alteration etc.

ELISA (Enzyme Linked Immunosorbent Assay): WHO recognized technique, will be provided to learn hands on for HIV-1 and HBV. The training will also include theory behind DNA sequence analysis and genetic profiling.

We believe that this opportunity provides everyone with a wide range of opportunities and trainees will get in-depth knowledge about modern techniques that would help individuals in seeking their career goals in research and diagnosis.



  1. I am a graduate of biotechnology and I beleive that the training will help me to upgrade my knowledge in the area of molecular and immunological techniques.Hence I will be happy if I get the opportunity.

    • Thank you for your interest in the training program. We will certainly upload details if any training program are to be conducted next time. Do check our blog and web-site.

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